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The built environment drives one-third of global biodiversity loss, yet the biodiversity impacts of individual building projects are neither measured nor mitigated. This critical gap presents an opportunity: incorporating biodiversity impact assessment into Life Cycle Assessment (LCA), a tool already widely adopted in the construction sector. LCA is currently used primarily to evaluate the climate impacts of building projects, while its potential for assessing biodiversity impacts remains largely untapped. In my doctoral thesis, I investigate the biodiversity impacts across the entire life cycle of a building and evaluate as well as develop the feasibility of the LCA framework for assessing biodiversity impacts in building projects. I explore how these impacts can be quantitatively assessed and examine the potential implications for the building design process.